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标题: 镇银膏对小鼠尾鳞片颗粒层形成的影响（山东医科大学） [打印本页]
作者: 见龙在田    时间: 2007-3-23 13:03
标题: 镇银膏对小鼠尾鳞片颗粒层形成的影响（山东医科大学）
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<>镇银膏药理学报告之二：镇银膏对小鼠尾鳞片颗粒层形成的影响（山东医科大学）</P></TD></TR>
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< ><FONT face="Times New Roman">1</FONT>．试验方法<FONT face="Times New Roman">  </FONT>取昆明种小鼠<FONT face="Times New Roman">60</FONT>只，体重<FONT face="Times New Roman">20</FONT>～<st1:chmetcnv UnitName="g" SourceValue="25" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on"><FONT face="Times New Roman">25g</FONT></st1:chmetcnv>，雌雄各半，每只小鼠均背部剃毛约<FONT face="Times New Roman"><st1:chmetcnv UnitName="cm" SourceValue="3" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on">3cm</st1:chmetcnv><SUP>2</SUP></FONT>，随机分为<FONT face="Times New Roman">6</FONT>组，第<FONT face="Times New Roman">1</FONT>组为空白对照组，于背部剃毛处涂抹芝麻油<FONT face="Times New Roman">0</FONT>．<FONT face="Times New Roman">05ml</FONT>／<st1:chmetcnv UnitName="g" SourceValue="10" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on"><FONT face="Times New Roman">10g</FONT></st1:chmetcnv>；第<FONT face="Times New Roman">2</FONT>～<FONT face="Times New Roman">4</FONT>组为镇银膏高、中、低剂量组，于背部剃毛处分别涂抹<FONT face="Times New Roman">0</FONT>．<FONT face="Times New Roman">1</FONT>、<FONT face="Times New Roman">0</FONT>．<FONT face="Times New Roman">05</FONT>和<FONT face="Times New Roman">0</FONT>．<FONT face="Times New Roman">025ml</FONT>／<st1:chmetcnv UnitName="g" SourceValue="10" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on"><FONT face="Times New Roman">10g</FONT></st1:chmetcnv>；第<FONT face="Times New Roman">5</FONT>组为去炎松组，于背部剃毛处涂抹去炎松软膏<FONT face="Times New Roman">0</FONT>．<FONT face="Times New Roman">0075mg</FONT>／<st1:chmetcnv UnitName="g" SourceValue="10" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on"><FONT face="Times New Roman">10g</FONT></st1:chmetcnv>；第<FONT face="Times New Roman">6</FONT>组为氨甲喋呤组，每只鼠<FONT face="Times New Roman">ip0</FONT>．<FONT face="Times New Roman">1mg</FONT>／<st1:chmetcnv UnitName="g" SourceValue="10" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on"><FONT face="Times New Roman">10g</FONT></st1:chmetcnv>，隔日<FONT face="Times New Roman">1</FONT>次，共<FONT face="Times New Roman">7</FONT>次。翌日脱颈椎处死小鼠，取尾部距尾根约<st1:chmetcnv UnitName="cm" SourceValue="2" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on"><FONT face="Times New Roman">2cm</FONT></st1:chmetcnv>处背面皮肤一长条，用<FONT face="Times New Roman">10</FONT>％福尔马林固定，石腊包埋，<FONT face="Times New Roman">HE</FONT>染色，在光学显徽镜下逐一观察每个小鼠的尾部鳞片，凡二个毛囊口之间的鳞片表皮有排列成行的颗粒层者，称为颗粒层的鳞片。计数每<FONT face="Times New Roman">100</FONT>个鳞片中有颗粒层的鳞片数，进行比较，并作统计学处理。</P>
< ><FONT face="Times New Roman">      2</FONT>．给药途径<FONT face="Times New Roman">  </FONT>皮肤</P>
< ><FONT face="Times New Roman">      3</FONT>．给药次数<FONT face="Times New Roman">  </FONT>隔日<FONT face="Times New Roman">1</FONT>次，共<FONT face="Times New Roman">7</FONT>次。</P>
< ><FONT face="Times New Roman">      4</FONT>．试验对照</P>
< ><FONT face="Times New Roman">     </FONT>（<FONT face="Times New Roman">1</FONT>）空白对照<FONT face="Times New Roman">  </FONT>芝麻油</P>
< ><FONT face="Times New Roman">     </FONT>（<FONT face="Times New Roman">2</FONT>）己知阳性药物对照及选择依据同前实验</P>
< ><FONT face="Times New Roman">      5</FONT>．试验结果见表<FONT face="Times New Roman">3 </FONT></P>
< ><FONT face="Times New Roman">               </FONT>表<FONT face="Times New Roman">3 </FONT>：<FONT face="Times New Roman"> </FONT>镇银膏对小鼠尾鳞片颗粒细胞层形成的影响</P>
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< ><FONT face="Times New Roman">     </FONT>组<FONT face="Times New Roman">      </FONT>别</P></TD>
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<P ><FONT face="Times New Roman"> </FONT>动<FONT face="Times New Roman">   </FONT>物<FONT face="Times New Roman">    </FONT>数</P></TD>
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<P >尾鳞片颗粒细胞层形成指数</P>
<P >（<FONT face="Times New Roman">x</FONT>±<FONT face="Times New Roman">s</FONT>）</P></TD></TR>
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<P >空白对照组</P>
<P >镇银膏高剂量组</P>
<P >镇银膏中剂量组</P>
<P >镇银膏低剂量组</P>
<P >去炎松软膏组</P>
<P >氨甲喋呤组</P></TD>
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<P ><FONT face="Times New Roman">10</FONT></P>
<P ><FONT face="Times New Roman">10</FONT></P>
<P ><FONT face="Times New Roman">10</FONT></P>
<P ><FONT face="Times New Roman">10</FONT></P>
<P ><FONT face="Times New Roman">10</FONT></P>
<P ><FONT face="Times New Roman"> 10</FONT></P></TD>
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<P ><FONT face="Times New Roman">7.86</FONT>±3<FONT face="Times New Roman">.48   ***</FONT></P>
<P ><FONT face="Times New Roman">19.00</FONT>±7<FONT face="Times New Roman">.02  ***</FONT></P>
<P ><FONT face="Times New Roman">17.76</FONT>±5<FONT face="Times New Roman">.75     </FONT></P>
<P ><FONT face="Times New Roman">8.99</FONT>±3<FONT face="Times New Roman">.25   </FONT></P>
<P ><FONT face="Times New Roman">7.41</FONT>±3<FONT face="Times New Roman">.35   *</FONT></P>
<P ><FONT face="Times New Roman">13.69</FONT>±4<FONT face="Times New Roman">.62</FONT></P></TD></TR></TABLE>
<P ><FONT face="Times New Roman">   </FONT>与空白对照组比较大<FONT face="Times New Roman">   *  P</FONT>＜<FONT face="Times New Roman">0. 05   ***  P</FONT>＜<FONT face="Times New Roman">0. 001<o:p></o:p></FONT></P>
<P >结果表明，镇银膏高、中剂量能非常明显的促进小鼠尾鳞片中颗粒层的形成（<FONT face="Times New Roman">P</FONT>＜<FONT face="Times New Roman">0. 001</FONT>），氨甲喋呤也有明显的促进颗粒层形成的作用（<FONT face="Times New Roman">P</FONT>＜<FONT face="Times New Roman">0. 05</FONT>），镇银膏低剂量作用不显著，与空白对照组比较无显著差异。</P></TD></TR></TABLE>



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