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标题: 镇银膏对小鼠外周血酸性非特异性酯酶（ANAE）的影响（山东医科大学） [打印本页]
作者: 见龙在田    时间: 2007-3-23 13:05
标题: 镇银膏对小鼠外周血酸性非特异性酯酶（ANAE）的影响（山东医科大学）
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<><FONT size=5>镇银膏药理学报告之三：镇银膏对小鼠外周血酸性非特异性酯酶（ANAE）的影响（山东医科大学）</FONT></P></TD></TR>
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< ><FONT size=5><FONT face="Times New Roman">    1</FONT>．试验方法取<FONT face="Times New Roman">BALB</FONT>／<FONT face="Times New Roman">C</FONT>小鼠<FONT face="Times New Roman">40</FONT>只，随机分为<FONT face="Times New Roman">4</FONT>组，体重<FONT face="Times New Roman">18</FONT>～<st1:chmetcnv UnitName="g" SourceValue="22" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on"><FONT face="Times New Roman">22g</FONT></st1:chmetcnv>，雌雄各半，每只鼠背部剃毛约<FONT face="Times New Roman"><st1:chmetcnv UnitName="cm" SourceValue="3" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on">3cm</st1:chmetcnv><SUP>2</SUP></FONT>，第<FONT face="Times New Roman">1</FONT>组为空白对照组于背部剃毛处涂抹<FONT face="Times New Roman">0</FONT>．<FONT face="Times New Roman">05ml</FONT>／<st1:chmetcnv UnitName="g" SourceValue="10" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on"><FONT face="Times New Roman">10g</FONT></st1:chmetcnv>芝麻油；第<FONT face="Times New Roman">2</FONT>～<FONT face="Times New Roman">4</FONT>组为镇银膏高、中、低剂量组，于背部剃毛处分别涂抹镇银膏<FONT face="Times New Roman">0</FONT>．<FONT face="Times New Roman">1</FONT>、<FONT face="Times New Roman">0</FONT>．<FONT face="Times New Roman">05</FONT>和<FONT face="Times New Roman">0</FONT>．<FONT face="Times New Roman">025ml/<st1:chmetcnv UnitName="g" SourceValue="10" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on">10g</st1:chmetcnv></FONT>，隔日<FONT face="Times New Roman">1</FONT>次，共<FONT face="Times New Roman">7</FONT>次。于最后给药次日由尾尖部取血涂片，吹干后接葛氏法在反应液中孵育<FONT face="Times New Roman">90min</FONT>，再以甲基绿复染<FONT face="Times New Roman">40min</FONT>。油镜下计数观察<FONT face="Times New Roman">100</FONT>个<FONT face="Times New Roman">T</FONT>淋巴细胞中<FONT face="Times New Roman">ANAE</FONT>染色阳性<FONT face="Times New Roman">T</FONT>淋巴细胞数，并按下列公式计算<FONT face="Times New Roman">ANAE<SUP>+</SUP></FONT>率（％）。</FONT></P>
< ><FONT size=5><FONT face="Times New Roman">                   ANAE <SUP>+</SUP>T</FONT>淋巴细胞数</FONT></P>
< ><v:line><FONT size=5></FONT></v:line><FONT size=5><FONT face="Times New Roman">   ANAE<SUP>+</SUP></FONT>率（％）<FONT face="Times New Roman">=                      </FONT>×<FONT face="Times New Roman">100%</FONT></FONT></P>
< ><FONT size=5><FONT face="Times New Roman">                    100</FONT>个<FONT face="Times New Roman">T</FONT>淋巴细胞</FONT></P>
< ><FONT size=5><FONT face="Times New Roman">     2</FONT>．给药途径<FONT face="Times New Roman">  </FONT>皮肤</FONT></P>
< ><FONT size=5><FONT face="Times New Roman">     3</FONT>．给药次数<FONT face="Times New Roman">  </FONT>隔日<FONT face="Times New Roman">1</FONT>次，共<FONT face="Times New Roman">7</FONT>次。</FONT></P>
< ><FONT size=5><FONT face="Times New Roman">     4</FONT>．试验对照（<FONT face="Times New Roman">1</FONT>）空白对照<FONT face="Times New Roman">  </FONT>芝麻油（<FONT face="Times New Roman">2</FONT>）己知阳性药物对照及选择依据，本试验未设阳性药物对照，因去炎松和氨甲喋呤均为己知的免疫抑制剂且本试验为作用机理的探讨，故未设阳性对照药物。</FONT></P>
< ><FONT size=5><FONT face="Times New Roman">     5</FONT>．试验结果见表<FONT face="Times New Roman">4</FONT></FONT></P>
< ><FONT size=5><FONT face="Times New Roman">          </FONT>表<FONT face="Times New Roman">4</FONT>：镇银膏对小鼠外周血<FONT face="Times New Roman">ANAE</FONT>的影响</FONT></P>
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<P ><FONT size=5>组<FONT face="Times New Roman">      </FONT>别</FONT></P></TD>
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<P ><FONT size=5><FONT face="Times New Roman">    </FONT>动<FONT face="Times New Roman">   </FONT>物<FONT face="Times New Roman">   </FONT>数</FONT></P></TD>
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<P ><FONT size=5><FONT face="Times New Roman">ANAE<SUP>+</SUP></FONT>率（％）</FONT></P>
<P ><FONT size=5>（<FONT face="Times New Roman">x</FONT>±<FONT face="Times New Roman">s</FONT>）</FONT></P></TD></TR>
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<P ><FONT size=5>空白对照组</FONT></P></TD>
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<P ><FONT face="Times New Roman" size=5>10</FONT></P></TD>
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<P ><FONT size=5><FONT face="Times New Roman">45.90</FONT>±6<FONT face="Times New Roman">.17  </FONT></FONT></P></TD></TR>
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<P ><FONT size=5>镇银膏高剂量组</FONT></P></TD>
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<P ><FONT face="Times New Roman" size=5>10</FONT></P></TD>
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<P ><FONT size=5><FONT face="Times New Roman">62.30</FONT>±5<FONT face="Times New Roman">.12  ***</FONT></FONT></P></TD></TR>
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<P ><FONT size=5>镇银膏中剂量组</FONT></P></TD>
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<P ><FONT face="Times New Roman" size=5>10</FONT></P></TD>
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<P ><FONT size=5><FONT face="Times New Roman">79.80</FONT>±7<FONT face="Times New Roman">.18  ***</FONT>△△</FONT></P></TD></TR>
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<P ><FONT size=5>镇鼠膏低剂量组</FONT></P></TD>
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<P ><FONT face="Times New Roman" size=5>10</FONT></P></TD>
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<P ><FONT size=5><FONT face="Times New Roman">56.10</FONT>±4<FONT face="Times New Roman">.70  **</FONT></FONT></P></TD></TR></TABLE>
<P ><FONT size=5><FONT face="Times New Roman">   </FONT>与空白对照组比较<FONT face="Times New Roman"> **  P</FONT>＜<FONT face="Times New Roman">0. 01   **  P</FONT>＜<FONT face="Times New Roman">0. 001   </FONT>中剂量与高、低剂量比较<FONT face="Times New Roman">  </FONT>△△<FONT face="Times New Roman">P</FONT>＜<FONT face="Times New Roman">0</FONT>．<FONT face="Times New Roman">01<o:p></o:p></FONT></FONT></P>
<P ><FONT size=5><FONT face="Times New Roman">    </FONT>结果表明，镇银膏高、中、低剂量均能非常显著提高小鼠外周血<FONT face="Times New Roman">T</FONT>淋巴细胞功能，<FONT face="Times New Roman">ANAE</FONT>活性增强，且中剂量效果最好，与高、低剂量组比较均有非常显著性差异（<FONT face="Times New Roman">P</FONT>＜<FONT face="Times New Roman">0. 01</FONT>）。</FONT></P></TD></TR></TABLE>



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