以下是引用[I]relax[/I]在2006-8-16 20:36:48的发言:[BR]<>估计你是没怎么看 uvn的帖子 自己搜索一下 仔仔细细的看看 里面有非常非常非常非常详细的uvn是什么原来 怎么回事:)
感谢7楼提醒,我查到:UVN也是一种紫外线疗法,目前在临床较常 见,其紫外线光谱组成为UVA 70%、UVB 14%、UVC 4%,其余为可见光,具有长波和中波 ...
实际上我想说的不是这, 难道我们所见到的紫外线治疗机就是单一的波长吗?稍有这方面知识的人都会否定这一点。同时还是想提醒大家,注意了吗,UVN中UVA 70%,这与有些文献的对银屑病治疗的有效波长UVB矛盾,哎,生在一个处处都存在陷阱的世界,我们不得不小心啊,包括下面我搜到的文献提纲(学术论文有多少可信性?),大家最好过过眼瘾,千万别中毒太深啊
UVN对角质形成细胞生长的抑制作用及其机制研究
唐玲1,于益芝2,顾军1,陶苏江1,张明徽2,王闻雅2,郑茂荣1,曹雪涛2
[摘 要] 目的 观察UVN(混合紫外线)对角质形成细胞(keratinocytes,KC)生长的抑制作用并分析其机制。方法 将角质形成细胞株HACAT经生理剂量的UVN照射后,通过LEICA显微镜观察其形态,通过四甲基偶氮唑盐[3(4,5dimethylthiazol2yl)2,5diphenyltetrazolium bromide,MTT]法检测其增殖情况,通过流式细胞术(FACS)分析UVN照射后不同时间HACAT的趋化因子受体CXCR2表达的改变。结果 生理剂量UVN照射HACAT后,部分细胞变圆,少量细胞脱落。照射后24h细胞的增殖受到明显抑制,此时其CXCR2的表达也明显降低,但至48h开始其增殖能力和CXCR2的表达开始恢复,72h后其增殖能力及CXCR2的表达进一步恢复,但与正常对照组间仍见差异。结论 UVN的照射可降低银屑病角质形成细胞CXCR2的表达,这可能因抑制了白细胞介素8(interleukin8,IL8)等细胞生长因子对KC的促增殖作用从而对银屑病表现出一定的治疗效果。
[关键词]银屑病;UVN;趋化因子受体
[中图分类号] R 594.8[文献标识码]A [文章编号]1001-7089(2001)06-0361-03
The Inhibitory Effect of UVN and Its Mechanism on Proliferation of Keratinocyte
TANG Ling,YU Yizhi,GU Jun,et al Department of Dermatology,Changhai Hospital,The Second Military Medical University,Shanghai 200433,China
Abstract: Objective To investigate the inhibitory effect of UVN and its mechanisms on proliferation of keratinocytes.Methods After the keratinocyte cell line HACAT was irradiated by UVN,the morphological characteristics of the cells were observed by LEICA microscope and the proliferative capacity was detected by MTT methods.The expression of CXCR2 was also assayed by flow cytometry.Results After UVN irradiation,few cells became round and quite a few cells looked like dead.The proliferative capacity and the CXCR2 expression on the cells also decreased significantly.But the most obvious decrease was detected at 24h after irradiation and then such alterations were reduced from 48h to 72h.Conclusion The downregulation of the CXCR2 expression in keratinocytes may contribute to the therapeutic effect of UVN irradiation on psoriasis as the overproliferation of keratinocytes in psoriasis of ten results from the combination of CXCR2 with its ligands such as interleukine8. |